Gas production from the Inner Otway Basin commenced in the early 2000s but the deep-water part of this basin remains an exploration frontier. Historically, the understanding of plays in this region were largely model driven and therefore the ground-truthing of depositional environments (DE) and gross depositional environments (GDE) are critical. This aspect has been investigated for the Sherbrook Supersequence (SS) by the integration of legacy wireline and core data, with regional 2D seismic facies mapping of new and reprocessed data from Geoscience Australia’s 2020 Otway Basin seismic program. Core observations were matched to wireline logs and seismic facies with resulting well based DE interpretations calibrated to seismic resolution Regional GDE intervals. Integration of well and seismic observations lead to the compilation of a basin-wide Regional GDE map for the Sherbrook SS. This GDE map indicates the distribution of Sherbrook SS play elements such as source rock, seal and reservoir, especially across the Deep Water Otway Basin where well data is sparse.